Plate Count Skim Milk Agar (PCA), 500 g

Solid medium for the plate count of milk and dairy products, according to DIN and FIL/IDF standards.
FORMULA* IN G/L :
Tryptone 5.00
Yeast extract 2.50
Skimmed milk 1.00
Dextrose 1.00
Agar 10.50
Final pH 7.0 ±0.2 at 25 °C
*Adjusted and/or supplemented as required to meet performance criteria.
DIRECTIONS :
Suspend 20 g of powder in 1 l of distilled water. Heat to boiling with constant stirring. Distribute into suitable containers and sterilize by autoclaving at 121 °C for 15 minutes.
DESCRIPTION :
This medium, after adding milk, is more nutritious than other standard media; however, the opalescence of the medium sometimes makes early observations difficult.